Haish, it's one of those weeks... (-_-)
On Monday my supervisor paid a surprise visit to CGAT and interrogated everybody on their research progress. Everyone had something to show for it; progress, results, optimization problems, etc.
Everyone but me.
Aku kena marah sebab result DNA extraction dan PCR tak keluar. Dah aku katakan dulu, Promega kit tu mungkin tak berkesan untuk extract DNA daripada tisu kepak kelawar. Aku minta beli brand Qiagen dia kata mahal. Memang pun. Siapa yang nak beli benda mahal kalau ada yang setengah harga...
Awalnya, aku ingat mungkin aku yang tersilap ikut manual. Something obviously went wrong somewhere. Aku cuba lagi. Tukar sana, betulkan sini; tak juga menjadi. Foto gel aku kosong. Cuma laddernya saja yang maha cantik. PCR tak payah cakap la. Lagi-lagi isu primer dimers. Bila supervisor minta results...
Hari ni aku gi quantify DNA dengan undergrad yang projek dan resultnya senasib dengan aku. Bacaan spectro dalam angka negatif. What does that mean? It means, percubaan kami untuk extract DNA kelawar menggunakan Promega kit telah gagal.
Gagal. Which also means: More labwork with time I don't have. Haish..
I feel like I got no place to go... Before, when things like this happened, I had shoulders to go crying on. People who can hear me out. There was always support. Everyone understands.
Yeah, there was lab then too... but then there were also coastlines and malls, forests and caves. We had surprise birthday parties, power lunches, bazaars, movies, late night beach visits and iftars under the open sky. We were students, but we were also presidents, managers, enterpreneurs, adventurers, superfoxes, brothers, sisters and housemates. We were always ready to go and be something..
Where did all that energy go? I miss it, and I certainly miss you much...
6 comments:
Oh my dearest K.Opie,I totally understand how you feel.Be strong K.Opie!Jgn pedulikan remark2 yang negatif dr orang.You do what you have to do with what you have in hands.Go,go K.Opie!
I'm having a little dispute at work la skrg ni..Sob..sob wish you were here!
saya ada tips untuk deal dengan supervisor bila tak dapat result.
1. Sblm jumpa make sure tahu dulu apa nak cakap. At least tahu lah apa jadi pada structure DNA bila time elongation, or sekurangnya pun, tahu ladder apa guna dan apa concentration gel yang perlu...
2. Cari kesalahan alat2.
3. Cari kesalahan reagen.
4. Sedikit muka innocent.
5. Dan tunjuk sifat mahu belajar.
Btw, supervisor yang pro takkan marah kalu tak dapat result, tapi tahu nak trobleshoot....
Kalu OD DNA negative itu, bukan DNA lah akak....
ingat, jangan cakap kita yang salah....hehehehe
Kak sofie, jgn give up lg! Ganbatte kudasai! I think every one of us are having moments like this all the time. It'll all pass soon enough...
Chaiyukk kak op!
Never gives up. Hold on till i come and rescue u. Cehh...lah nnt kak op plak yg rescue sy.
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